On Monday, union members at ambulance trusts in the West Midlands, North West, South Central, South Coastal, and East Midlands had planned to strike.
However, the Unite union paused the strike action on Sunday afternoon in order to enter pay talks with the Government.
Unite head of operations Gail Cartmail said: “Following further assurances from the Government over the weekend, Unite has in good faith agreed to pause the strike action.
“If the meeting doesn’t meet these assurances strike action will resume.”
It would have marked the first time that Unite members in South Central and South Coastal had taken industrial action.
Members in Yorkshire, who are also planning to strike for the first time, were due to take industrial action on Wednesday.
Unite had also called off strikes in Wales on March 6 due to pay talks continuing with the Welsh Government.